Evaluation of withania somnifera in a middle cerebral artery occlusion model of stroke in rats

New withanolides and other constituents from the fruit of Withania somnifera. ginseng) and ashwagandha (Withania somnifera) in rats: a safety assessment. Withania somnifera in a middle cerebral artery occlusion model of stroke in rats. New withanolides and other constituents from the fruit of Withania somnifera. ginseng) and ashwagandha (Withania somnifera) in rats: a safety assessment. Withania somnifera in a middle cerebral artery occlusion model of stroke in rats. Chaudhary G, Sharma U, Jagannathan NR, Gupta YK () Evaluation of Withania somnifera in a middle cerebral artery occlusion model of stroke in rats.

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This service is more advanced with JavaScript available, learn more at http: Molecular Neurobiology. Its protective effect has been elucidated in many neurodegenerative pathologies, although there is a paucity of data regarding its effects in ischemic stroke. We examined the neuroprotective properties of an aqueous extract of WS in both pre- and poststroke treatment regimens in a mouse model of permanent distal middle cerebral artery occlusion pMCAO. These results indicate the interplay of antioxidant-antiapoptic pathways and the possible involvement of angiogenesis itty bitty alan jackson the protective mechanism of WS while emphasizing the noninvolvement of one of the prime pathways of neurogenesis.

Our results suggest that WS could be a potential prophylactic as well as a therapeutic agent aiding stroke repair, and that part of its mechanism could be attributed to its antiapoptotic and antioxidant properties. Skip to main content. Advertisement Hide.

Article First Online: This is a preview of subscription content, log in to check access. Circulation Ann Neurol Wermuth CG Multitargeted drugs: Drug Discov Today 9: Altern Ther Health Med 8: Neurochem Res Neuroscience Prog Neuropsychopharmacol Biol Psychiatry Phytother Res Phytomedicine 8: Biochem Biophys Res Commun Hum Exp Toxicol Cell Mol Biol Lett J Med Food Br J Pharmacol Clin Nutr evaluation of withania somnifera in a middle cerebral artery occlusion model of stroke in rats Clin Exp Pharmacol Physiol PLoS One 7: Free Radic Biol Med J Neurochem Front Mol Neurosci 4: Trends Biochem Sci Blood Part two: Open Neurol J 4: Cancer Chemother Rep Part 1 Behav Ecol J Clin Neurosci: Off J Neurosurg Soc Australas Love S Oxidative stress in brain ischemia.

Brain Pathol 9: Dore S Decreased activity of the antioxidant heme oxygenase enzyme: J Biol Chem Nat Med 6: Mol Neurobiol Stroke Clin Cancer Res Biochimie J Cell Biol J Cell Biochem J Cereb Blood Flow Metab Chaitanya GV, Babu PP Multiple apoptogenic proteins are involved in the nuclear translocation of apoptosis inducing factor during transient focal cerebral ischemia in rat. Brain Res Evaluation of withania somnifera in a middle cerebral artery occlusion model of stroke in rats Neurosci Cell Signal Science Trends Pharmacol Sci Carmichael ST Themes and strategies for studying the biology of stroke recovery in the poststroke epoch.

Curr Cardiol Rev 6: Neurobiol Dis Cell Mol Neurobiol 49 2: Exp Cell Res Development Curr Opin Neurobiol Curr Signal Transduct Ther 7: Front Neurosci 5: Front Cell Neurosci 7: Prog Neurobiol Nat Rev Neurosci Functions and mechanisms of beta-catenin-independent Wnt signaling. Dev Cell 5: Genes Dev PLoS One 8: Mol Cell Biol Shah 1 Email author 1.

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Evaluation of Withania somnifera in a middle cerebral artery occlusion model of stroke in rats.

Skip to main content. Log In Sign Up. Evaluation of Withania somnifera in a middle cerebral artery occlusion model of stroke in rats Clinical and Experimental Pharmacology and Physiology, Yogendra Gupta. Evaluation of Withania somnifera in a middle cerebral artery occlusion model of stroke in rats.

Because W. Stroke causes brain injury in millions of people world- could be due to its anti-oxidant effect. The present study wide each year. Despite the enormity of the problem, there is provides first evidence of the effectiveness of an Indian evaluation of withania somnifera in a middle cerebral artery occlusion model of stroke in rats in currently no approved therapy that can reduce infarct size or focal ischaemia.

One of the approaches that can be used Key words: Ischaemic stroke is a leading cause of death and long-term dis- 2. Two groups of male Wistar rats were pretreated with a ability worldwide, yet no satisfactory treatment is available.

The hydroalcoholic extract of W. Thereafter, rats were subjected to focal ischaemia by activator and only in a few selected patients. After 2 h protective agents, despite proving effective in animal models of MCA evaluation of withania somnifera in a middle cerebral artery occlusion model of stroke in rats, reperfusion was allowed by retracting the stroke, have largely failed to fulfil their promise in clinical trials thread.

Animals were assessed for ischaemic changes using and, as such, currently there is no neuroprotective drug that is being diffusion-weighted imaging 30 min after reperfusion. Twenty- used clinically. Combining neuroprotective drugs with thrombolytics has marker of oxidative stress malondialdehyde MDA.

The demonstrated encouraging results in animal models but, as yet, control group received vehicle and a similar protocol was there are no results available from clinical trials.

Significant motor impairment, with elevated levels of melatonin and meloxicam shows better effects compared with the MDA, was observed in vehicle-treated MCA-occluded rats.

In two drugs used alone. Treatment with W. Further suggestions from clinical trials have did not improve motor performance or decrease the elevated shown that very early treatment after stroke may be necessary for levels of MDA. However, when the pretreatment time of the drugs to be effective.

In evaluation of withania somnifera in a middle cerebral artery occlusion model of stroke in rats W. The agent to be 30 days -pretreated group, the percentage hemispheric lesion used prophylactically should be efficacious, safe, vignette design vector adminis- area in diffusion-weighted imaging was significantly attenu- tered and affordable.

These are currently be re-evaluated by extensive research on different plant species and their therapeutic principles. Because herbal drugs have a relatively higher therapeutic window, fewer side-effects and are economical, they have gained lot of acceptance in recent years and are potential Correspondence: Delhi,India.

The rat was placed on the string at a point midway between the supports and evaluated according to the following scale: The 5, escapes. On the day of and natural dark and light cycles. Rats were allowed free access to standard dry before MCA occlusion, rats were conditioned to the accelerating rota rod rat diet Golden Feeds, Delhi, India and tap water. All procedures described Ugo Basile, Comerio, Italy. Each animal received a training session on the were reviewed and approved by the Institutional Animal Ethics Committee.

Each rat then received a single baseline trial on the accelerating rota rod in which the Drug schedule spindle increased in speed from 4 to 40 r. At Rats were divided into four groups: Each Foot fault test group consisted of 12 animals. Rats were placed on an elevated grid floor with a mesh size of 30 mm for A standardized hydroalcoholic extract of W.

After acclimatization, rats were placed on onpayskiosk firefox grid for from Dabur Research Foundation, Ghaziabad, India.

The weight of the rats was recorded every day before drug counted. On 16th and 31st days in the two groups given W. The rats. The percentage error was determined as follows: In the sham-operated group, rats were operated on and the filament occluding the MCA was pulled within 60 s. Experiments were performed at 4. Briefly, the right common carotid artery and polarized birdcage volume resonator. The 2 mm slice thickness was Johnson, Aurangabad, Indiawith its tip rounded by heating quickly near used because decreasing the slice thickness would have decreased the signal a flame, was advanced from the external carotid artery into the lumen of the to noise intensity ratio and, in order to increase it, the number of averages evaluation of withania somnifera in a middle cerebral artery occlusion model of stroke in rats carotid artery until the resistance was felt approximately 17 mm, would have to be increased, thereby increasing the acquisition time.

After which ensures that the suture entered the anterior cerebral artery, thus identification of the area microsoft office 2010 web apps interest, diffusion-weighted images were occluding the origins of the anterior cerebral artery, the MCA and the acquired using a stimulated echo diffusion-weighted pulse sequence.

The nylon filament was allowed to remain Diffusion-weighted images were acquired with the following acquisition in place for 2 h. After 2 h, the filament was retracted so as to allow parameters: Contrast of images of diffusion-weighted images were adjusted using the grey scale.

However, when W. Measurement of lipid peroxidation Effect of W. Acetic acid 1. In the sulphate 0. The mixture was cooled W. The mixture was shaken vigorously.

On increasing the period of pretreatment of centrifugation at g for 10 min, the organic layer was withdrawn and W. The bodyweight of rats in both groups was noted each Effect of W. The mean weight gain after 15 and 30 days pretreatment in the Withania somnifera-treated groups was not significantly different Middle cerebral artery occlusion significantly increased the levels compared with the vehicle-treated MCA-occluded rats.

In the weight in the sham-operated, vehicle-treated MCA-occluded and W. However, in the W. The effect of W. The neurological score significantly Effect of W. MDA, malondialdehyde. Focal ischaemia was seen in all vehicle-treated MCA-occluded compared with the contralateral side.

The mean value of signal rats. However, Chinese herbal preparations, such as radix multiorhizzae, tetramethylpyrazine, tetrandrine and ginseng, have been studied extensively in cerebral ischaemia. Moreover, recurrence is a very prevalent phenomenon seen in patients who had suffered from one episode of stroke.

Therefore, the use of a prophylactic approach becomes important in such patients. Aspirin has been commonly used as for the secondary prevention of stroke due to its antiplatelet properties. Although aspirin is cheap and effective, its side-effect profile e. We have recently demonstrated the effectiveness of prophylactic treatment of trans-resveratrol, a potent anti-oxidant derived from red wine, in preventing neurological damage caused by occlusion of the MCA in rats.

Because recirculation occurs frequently after cerebral ischaemia, to resemble this clinical setting, ischaemia— reperfusion was induced in rats by mechanical clipping of the MCA or photothrombotic occlusion of the araketu as melhores de roberto. However, the limi- tation of these models is that they involve craniotomy.

Table 2 Signal intensity measured by diffusion-weighted imaging in vehicle- and Withania somnifera-pretreated middle cerebral artery occluded rats Signal intensity Contralateral Ipsilateral Fig. Effect of Withania somnifera in stroke It was observed that 2 h MCA occlusion followed by reperfusion complex process involving various neurotransmitters and enzymes, decreased the motor performance, as evidenced by a decrease in the such as glutamate, calcium, nitric oxide and cyclo-oxygenase, rota rod performance test and spontaneous locomotor activity and inhibition of these neurotransmitters and enzymes by W.

There was also an increase in the The present study provides, to the best of our knowledge, levels of MDA, indicating oxidative stress. The ischaemia was the first experimental evidence suggesting a potential benefit confirmed using diffusion-weighted imaging, which showed of W. The present study indicates that there could be herbs that occluded. The hyperintensity seen in the MCA-occluded rats may need to be explored as potential treatments for stroke.

The accumulation of water in cells results in reduced water molecule sibirska pesma itunes and the region appears hyperintense.

Kaste M. Thrombolysis in ischemic stroke: Present and future. Role of combined therapy. Current status and perspectives of neuro- with a hydroalcoholic extract of W. A hydroalcoholic protection in ischemic stroke treatment. Enhanced protection by melatonin alcoholic extracts. Albers GW. Rationale for early intervention in acute stroke. When the pretreatment period of W. Prophylactic neuroprotection 30 days, a significant improvement in the motor performance test for cerebral ischemia. Stroke ; This implies that the region controlling motor 6.

Anxiolytic- coordination caudate putamen as well as the cortex was less antidepressant activity of Withania somnifera glycowithanolides: An affected in W. Phytomedicine ; 7:

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